Less than 1 percent of the initial cells fuse to form hybridoma cells. Unused B cells in the culture stop dividing naturally, while chemotherapy destroys the unfused myeloma cells. Researchers use HAT ...

This hybrid cell line had the antibody-producing ability of a B cell ... He and a friend sat in a rowboat talking about a hybridoma paper they had just read. The discovery of monoclonal antibodies and ...

Building on this method, Genetix has developed a technology that traps, detects and measures the relative secretion of monoclonal antibody from the hybridomas (or transfected cells such as myelomas).

Antibody-secreting hybridoma cells labeled with eFL450 (Blue) were mixed with target cells expressing a specific antigen that had been labeled with eFL670 (Far Red). This enabled the detection of ...