Less than 1 percent of the initial cells fuse to form hybridoma cells. Unused B cells in the culture stop dividing naturally, while chemotherapy destroys the unfused myeloma cells. Researchers use HAT ...

Similarly, incubating cells in microliter volumes requires significant time to accumulate sufficient antibody concentration to detect the titer ... leads using the hybridoma approach.

By day 28, the titer of specific antibodies is already ... We also tested the adjuvant for immunizing mice for hybridoma development. Mice were injected with E7 recombinant protein of HPV16 ...