This Research Topic is the second volume of the “Unraveling Breast Cancer Complexity: Insights from Single-Cell Sequencing and Spatial Transcriptomics” ...

Single-cell RNA transcriptomics allows researchers to broadly profile the gene expression of individual cells in a particular tissue. This technique has allowed researchers to identify new subsets of ...

The liver is remarkable for its ability to regenerate after injury, yet when this process fails, acute liver failure (ALF) carries devastating outcomes. Traditional research methods, reliant on bulk ...

Spatial transcriptomics (ST) technologies reveal the spatial organization of gene expression in tissues, providing critical insights into development, neurobiology, and cancer. However, the high cost ...

Initially, cells are dissociated from liver tissues into single-cell suspensions using in vivo enzymatic perfusion or ex vivo digestion methods. The cells of interest are then enriched from the ...

Breast cancer remains one of the most prevalent and life-threatening forms of cancer, impacting millions worldwide. This malignancy's heterogeneity and complexity have long posed significant ...