In most images it needs to be in focus. Depth of field Everything in this area appears in focus. When this distance is large the depth of field is said to be wide or deep. When it's small the ...

Under traditional imaging, large portions of the algae lie beyond the depth of field of the 20x/0.5NA objective and thus appear blurred. By placing a cubic phase mask in the back aperture of the ...

It can be hard to keep track of all of them. Today, we want to go over one that gets used a lot: shallow depth of field. We'll review the definition, look at some examples, and talk about why ...