Note: An expanded version of this article, with complete Methods and Materials, is available exclusively in Web Extras. Quantitative reverse transcriptase (RT) PCR has evolved into a widely used tool ...
1| Combine the following components of the master mix on ice and mix thoroughly: 10× KLA reaction buffer (pH 9.2): 500 mM Tris base, 160 mM (NH 4) 2 SO 4, 25 mM MgCl 2, 1% Tween 20. Note, the pH is ...
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