The medium is a high protein formulation for maximizing cell growth, and it is ready-to-use and needs only the addition of L-Glutamine. DCCM-1 contains BSA. DCCM-2: A serum-free medium, designed for ...
Freezing medium: 90% fetal calf serum, 10% DMSO (sterilized ... The plating density of MEFs required will vary from human embryonic stem cell line to cell line. We use between 2 × 10 5 and ...