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New drug combination shows promise in treating melanoma brain metastasis
A federally funded research team led by Sheri Holmen, PhD, investigator at Huntsman Cancer Institute and professor in the ...
Novel oral drug therapy combination shows promise for advanced melanoma patients
A research team led by Sheri Holmen, Ph.D., investigator at Huntsman Cancer Institute and professor in the Department of ...
Science Daily1d
New CRISPR tool enables more seamless gene editing -- and improved disease modeling
Advances in the gene-editing technology known as CRISPR-Cas9 over the past 15 years have yielded important new insights into the roles that specific genes play in many diseases. But to date this ...
Investigating the 'glue' that holds cells together and allows them to communicate
Northwestern Medicine investigators have uncovered new insights into how intercellular "glue" functions to enable ...
News Medical1mon
How to analyze, sort and dispense single cells with picodroplets
Antibody-secreting hybridoma cells labeled with eFL450 (Blue ... This study involved a CHO cell line secreting human IgG4κ antibody being mixed with a combination of: Cyto-Cellect ® (Sphere ...
Frontiers10mon
Development of novel monoclonal antibodies for blocking NF-κB activation induced by CD2v protein in African swine fever virus
Splenocytes were fused with Sp2/0 myeloma cells using PEG 1500. Positive hybridomas were subcloned and high-producing lines were injected into mice to induce ascites. Purification was achieved using a ...
Macmillan Cancer Support1y
Head and neck cancer
About 9 out of 10 head and neck cancers (90%) start in squamous cells. Squamous cells line the surfaces inside the head and neck, such as the mouth, nose and throat. The most common place for head and ...
Frontiers3y
Robust Expression of Functional NMDA Receptors in Human Induced Pluripotent Stem Cell-Derived Neuronal Cultures Using an Accelerated Protocol
We successfully converted 3 separate iPSC-derived NPC lines to mature ... was purchased from Cell Signaling Technology (Danvers, MA, United States). Synaptotagmin-1 antibody was purchased from ...
eLife5y
Toxoplasma gondii infection drives conversion of NK cells into ILC1-like cells
Innate lymphoid cells (ILCs) were originally classified based on their cytokine profiles, placing natural killer (NK) cells and ILC1s together, but recent studies support their separation into ...
eLife6y
A toolbox of IgG subclass-switched recombinant monoclonal antibodies for enhanced multiplex immunolabeling of brain
We cloned immunoglobulin G (IgG) variable domains from cryopreserved hybridoma cells and input them into an integrated pipeline for expression and validation of functional R-mAbs. To improve ...