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Video: DNA-affinity-purified Chip DAP-chip Method to Determine Gene Targets for Bacterial Two component Regulatory Systems
17.0K Views. Lawrence Berkeley National Laboratory. The overall goal of the following experiment is to determine genomic binding sites of bacterial two component response regulators using an in vitro microarray based assay. This is achieved by cloning and purifying a tagged response regulator protein, activating it by in vitro phosphorylation ...
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